Jasmine Asy Syauqi Ramadhani, Yasmin Nurfitriyanti, Maedia Salsabilla, Naurah Bagia Aryani?, Rifqi Muhammad Hajid?
Prodi Farmasi Fakultas Kedokteran Universitas Negeri Semarang Semarang, Jawa Tengah

Abstract

Secondary metabolites are compounds that are the result of biosynthesis derived from primary metabolites, and are generally produced by organisms to protect themselves from the environment and from attacks by other organisms. Vis spectrophotometry (vis spectrophotometry) is an analytical technique used to measure the absorbance of light by a sample at a certain wavelength in the visible light range (vis). This study aims to analyze various secondary metabolites in natural materials using Vis spectrophotometry. Secondary metabolites such as flavonoids, terpenoids, and alkaloids have an important role in plant defense and provide various health benefits for humans. Vis spectrophotometry is used to identify and measure the concentration of these metabolites based on their absorbance characteristics at certain wavelengths. The results of the study showed that the Vis spectrophotometry method is able to provide accurate identification and efficient quantification of secondary metabolites in various natural material samples. Flavonoids, terpenoids, and alkaloids were successfully identified and quantified using this technique. This study also confirms that Vis spectrophotometry is a very useful tool in chemical analysis and has wide applications in the pharmaceutical, nutritional, and cosmetic industries. Overall, this study provides a deep insight into the use of Vis spectrophotometry in secondary metabolite analysis and shows the potential of this method for further research and practical applications in various fields.
Keywords: Secondary Metabolites, Natural Products, Vis Spectrophotometry